Download CBSE 2009 Class Xll Biotechnology Sample Papers

Biotechnology is the field that has been made after the collaboration of the two fields viz. Biology and technology/engineering. It has been found as one of the fastest growing field in the world scenario. Sometimes, conflicts occur that Biotechnology and Genetic Engineering are similar fields but that’s not the case. The two fields have regarded as totally separate fields.

Download CBSE 2009 Class Xll Biotechnology Sample Papers

• Biotechnology Sample Paper 1
• Biotechnology Sample Paper 2
• Biotechnology Sample Paper 3
Examination Pattern:

• There is a one time paper conducted which is of duration of three hours.
• There are total of 70 questions to be attempted in the examination.

Syllabus:

Unit : Protein and Gene Manipulation Marks 40

Chapter I: Protein Structure and Engineering (15 Marks)

• Introduction to the world of Proteins
• 3-D Shape of Proteins
• Structure Function relationship in Proteins
• Purification of Proteins
• Characterization of Proteins
• Protein based products
• Designing Proteins
• Proteomics

Chapter II: Recombinant DNA Technology (15 Marks)

• Introduction
• Tools of rDNA Technology
• Making Recombinant DNA
• DNA Library
• Introduction of Recombinant DNA into host cells
• Identification of recombinants
• Polymerase Chains Reaction (PCR)
• DNA Probes
• Hybridization Techniques
• DNA Sequencing
• Site-directed mutagenesis

Chapter III: Genomics and Bioinformatics (10 Marks)

• Introduction
• Genome Sequencing Projects
• Gene Prodiction and counting
• Genome similarity, SNP’s and comparative genomics
• Functional Genomics
• History of Bioinformatics
• Sequences and Nomenclature
• Information Sources
• Analysis using Bioinformatics tools

Unit : Cell Culture Technology (30 Marks)

Chapter I: Microbial Culture and Applications

• Introduction
• Microbial Culture Techniques
• Measurement and Kinetics of microbial Growth
• Scale up of microbial process
• Isolation of microbial products
• Strain isolation and Improvement
• Applications of microbial culture technology
• Bioethics in microbial technology

Chapter II: Plant Cell Culture and Applications

• Introuction
• Cell and Tissue Culture Techniques
• Applications of Cell and Tissue Culture
• Gene Transfer Methods in Plants
• Transgenic Plants with Beneficial Traits
• Diagnostics in Agriculture and Molecular Breeding
• Bioethics in Plant Genetic Engineering

Chapter III: Animal Cell Culture and Applications 10 Marks

• Introduction
• Animal Cell Culture Techniques
• Characterisation of Cell Lines
• Scale-up of Animal Culture Process
• Applicationsof Animal Cell Culture
• Stem Cell Technology
• Bioethics of Genetic Engineering in Animals

Practicals

Note: Every student will be required to do the following experiments during the academic session

List of Experiments

• Isolation of bacterial plasmid DNA and its detection by gel electrophoresis
• Restriction digestion of plasmid DNA and its analysis by gel electrophoresis
• Bacterial transformation using any plasmid
• Data retrieval and data base search using internet site NCBI
• Download a DNA and protein sequence from internet, analyze and comment on it.
• Cell viability assay (using Evans blue Stain)
• Determination of blood groups.
• Estimation of DNA
• Ion-exchange chromatography for proteins.
• Reading of a DNA sequencing gel and arrive at the sequence.
• Estimation of blood glucose by enzymatic method (GOD/POD)
• Project work.

Scheme of Evaluation:

Time: 3 Hours Max. Marks 30
The scheme of evaluation at the end of the session will be as under:
A. Two experiments : 6+6 (only one computer based practical)
Practical record : 04
Viva on Practical : 04
B. Project work :
Write up : 05
Viva on project : 05
Total 30

Recommended Books:

A Textbook of Biotechnology-Class XI: published by CBSE, New Delhi.
A Laboratory Manual of Biotechnology-Class XI: published by CBSE, New
Delhi.
A Textbook of Biotechnology-Class XII: published by CBSE, New Delhi
A Laboratory Manual of Biotechnology- Class XII: published by CBSE, New
Delhi.